Assignment: "Learning" Sept. 1 - 15

[Rob Adams]

Originally Posted by kailacia

Learning to Paint:



Exif:
Canon EOS Rebel T1i
Exposure: 0.04 sec (1/25)
Aperture f/5.0
Focal Length 39 mm
ISO Speed 1600
Flash Off, Did not fire
Date and Time (Original) 2010:09:09 04:17:40
ISO 100

Brilliant!! I love it

[Rob Adams]

Originally Posted by nels141

Today was my son's first day of school.



Exif Info:

Taken on September 14, 2010 at 2.27pm PDT (edit)

Exif data
Camera Nikon D200
Exposure 0.017 sec (1/60)
Aperture f/4.0
Focal Length 18 mm
ISO Speed 100

Edited in Photoshop

Cute...a great shot

[mrteacherdude]

Originally Posted by riaflor_alcabedos

yes, you're right about the control and the treated ones =)
the difference just lies on the size/weight of the uterus =)

the treated rats (w/out ovaries) with higher dosage of estrogen have the same or nearly the same size/weight as the control (w/ ovaries; not injected with estrogen) while those injected with lower dosage of estrogen have smaller uterus size/lesser weight than the control.

So you were able to determine normal estrogen levels based on varying injection amounts and comparing to the controls? Or were you just looking at it from a more qualitative perspective?

Sorry to all if it seems as if I'm hijacking the thread. It's not my intentions, just curious about the experiment.

[riaflor_alcabedos]

Originally Posted by mrteacherdude

So you were able to determine normal estrogen levels based on varying injection amounts and comparing to the controls? Or were you just looking at it from a more qualitative perspective?

The experiment is performed basically to measure the activity of estrogen or its effects specifically to the ovariectomized rats. Estrogen administration exhibits effects on the endometrium (inducing endometrial mitosis), thus increasing the height of the lining epithelium; increasing blood supply, and so on. As a result, the uterus increases in weight and size (even if the rats do not have ovaries). So this part is the quali part.

But quantitative analysis can also be done by plotting the weight of the uteri against the dose of estrogen administered to produce a standard dose-response curve which will be compared to the standard dose-response curve.

We are not yet done with the quanti part though.

[mrteacherdude]

Originally Posted by riaflor_alcabedos

The experiment is performed basically to measure the activity of estrogen or its effects specifically to the ovariectomized rats. Estrogen administration exhibits effects on the endometrium (inducing endometrial mitosis), thus increasing the height of the lining epithelium; increasing blood supply, and so on. As a result, the uterus increases in weight and size (even if the rats do not have ovaries). So this part is the quali part.

But quantitative analysis can also be done by plotting the weight of the uteri against the dose of estrogen administered to produce a standard dose-response curve which will be compared to the standard dose-response curve.

We are not yet done with the quanti part though.

Sounds like fun! I like it
Even though I'm quite intrigued, I won't ask any more questions now because this isn't really the appropriate place to do so, and I'm sure you're quite busy.Thanks for the responses though. Have fun!