I generally shoot program mode so I can shift shutter speed and aperture at will, and use spot metering, manual focus, and always manual ISO. I shoot at 100 (my camera's lowest), and only go up from there if there's a need or if I'm experimenting.
Because of shooting film until recently, I'm usually comfortable about raising to 200, and wary of shooting at 400. But raising the ISO at all is a pretty rare occasion for me.
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